China Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit baetsi le barekisi |Yinye

Novel Coronavirus (SARS-CoV-2) Multiplex Real-Nako PCR Kit

Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit Setšoantšo se Hlahelitsoeng

Tlhaloso e Khutšoanyane:

REF 500190 Tlhaloso 96 Liteko / Lebokose

Molao-motheo oa ho lemoha PCR Mehlala Nasal / Nasopharyngeal swab

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

PCR kit ena e hlokolosi haholo, e seng e loketse ho sebelisoa e fumaneha ka mokhoa oa lyophilized (mokhoa oa ho omisa ka leqhoa) bakeng sa polokelo ea nako e telele.Thepa e ka tsamaisoa le ho bolokoa mocheso oa kamore 'me e tsitsitse selemo se le seng.Tube e 'ngoe le e' ngoe ea premix e na le li-reagents tsohle tse hlokahalang bakeng sa ho holisa PCR, ho kenyeletsoa Reverse-transcriptase, Taq polymerase, primers, probes, le dNTPs substrates.E hloka feela ho eketsa metsi a hloekisitsoeng a 13ul le template ea 5ul e nkiloeng ea RNA, ebe e ka tsamaisoa le ho holisoa lisebelisoa tsa PCR.

Mochini oa qPCR o lokela ho kopanalitlhoko tse latelang:

1. Fit 8 strip PCR tube volume 0,2 ml
2. Ho ba le mecha e fetang 4 ea ho lemoha:

Channel	Ho hlasimolla (nm)	Phallo (nm)	Li-dyes tse lekantsoeng pele
1.	470	525	FAM, SYBR Green I
2	523	564	VIC, HEX, TET, JOE
3.	571	621	ROX, TEXAS-RED
4	630	670	CY5

Li-platform tsa PCR:
7500Real-Time PCR System, Biorad CF96, iCycler iQ™ Real-Time PCR Detection System, Stratagene Mx3000P, Mx3005P

Bothata ba ho tsamaisa ketane e batang eaNovel Coronavirus Nucleic Acid Section reagent

Ha li-reagents tse tloaelehileng tsa ho lemoha li-nucleic acid li tsamaisoa sebakeng se selelele, (-20±5) ℃ polokelo le lipalangoang tse batang lia hlokahala ho netefatsa hore bioactive ea enzyme ho li-reagents e lula e sebetsa.Ho etsa bonnete ba hore mocheso o fihla boemong bo tloaelehileng, ho hlokahala lik'hilograma tse 'maloa tsa leqhoa le omileng bakeng sa lebokose le leng le le leng la nucleic acid test reagent esita le ka tlase ho 50g, empa e ka nka matsatsi a mabeli kapa a mararo feela.Ka pono ea ts'ebetso ea indasteri, boima ba 'nete ba li-reagents tse fanoeng ke bahlahisi bo ka tlase ho 10% (kapa ka tlase ho boleng bona) ba setshelo.Boholo ba boima bo tsoa ho leqhoa le omileng, lipakete tsa leqhoa le mabokose a foam, kahoo litšenyehelo tsa lipalangoang li phahame haholo.

Ka Hlakubele 2020, COVID-19 e ile ea qala ho ata ka bongata kantle ho naha, mme tlhoko ea Novel Coronavirus nucleic sensor reagent e ile ea eketseha haholo.Ho sa tsotellehe litšenyehelo tse phahameng tsa ho romela li-reagents ka har'a ketane e batang, bahlahisi ba bangata ba ntse ba ka e amohela ka lebaka la bongata bo bongata le phaello e phahameng.

Leha ho le joalo, ka ntlafatso ea maano a naha a thomello ea thepa bakeng sa lihlahisoa tse khahlanong le seoa, hammoho le ntlafatso ea taolo ea naha holim'a phallo ea batho le thepa, ho na le katoloso le ho hloka botsitso nakong ea lipalangoang tsa li-reagents, e leng se bakileng mathata a hlahelletseng a lihlahisoa. ka lipalangoang.Nako e atolositsoeng ea lipalangoang (nako ea lipalangoang e ka bang halofo ea khoeli e tloaelehile haholo) e lebisa ho hloleheng ha sehlahisoa khafetsa ha sehlahisoa se fihla ho moreki.Sena se tšoentse likhoebo tse ngata tse romellang li-nucleic acid.

Theknoloji ea Lyophilized bakeng sa reagent ea PCR e thusitse lipalangoang tsaNovel Coronavirus nucleic acid e lemoha reagent lefatšeng ka bophara

Li-reagents tsa PCR tsa lyophilized li ka tsamaisoa le ho bolokoa mocheso oa kamore, o ke keng oa fokotsa litšenyehelo tsa lipalangoang feela, empa hape o qoba mathata a boleng a bakoang ke ts'ebetso ea lipalangoang.Ka hona, lyophilizing reagent ke tsela e molemohali ea ho rarolla bothata ba lipalangoang tsa kantle ho naha.

Lyophilization e kenyelletsa ho hatsetsa tharollo hore e be boemong bo tiileng, ebe o nyenyefatsa le ho arola mouoane oa metsi tlas'a boemo ba vacuum.Solute e omisitsoeng e sala ka har'a setshelo se nang le sebopeho le mosebetsi o tšoanang.Ha ho bapisoa le li-reagents tse tloaelehileng tsa mokelikeli, karolo e felletseng ea lyophilized Novel Coronavirus nucleic acid e lemohang reagent e hlahisoang ke Liming Bio e na le litšobotsi tse latelang:

Ho tsitsisa mocheso o matla haholo:e ka khona ka kalafo ea ho ema ho 56 ℃ bakeng sa matsatsi a 60, 'me morphology le ts'ebetso ea reagent li lula li sa fetohe.
Ho boloka mocheso le lipalangoang tse tloaelehileng:ha ho hlokahale hore ho be le ketane e batang, ha ho hlokahale hore u boloke mocheso o tlaase pele u ka koala, lokolla ka ho feletseng sebaka sa polokelo e batang.
E se e loketse ho sebelisoa:lyophilizing ea likarolo tsohle, ha ho hlokahale hore ho hlophisoe tsamaiso, ho qoba ho lahleheloa ke likarolo tse nang le viscosity e phahameng joalo ka enzyme.
Multiplex targets ka tube e le 'ngoe:Sepheo sa ho sibolloa se akaretsa mofuta oa "coronavirus" oa ORF1ab, N gene, S gene ho qoba ho fetoha ha vaerase.E le ho fokotsa bobe ba bohata, mofuta oa RNase P oa motho o sebelisoa e le taolo ea ka hare, e le ho finyella tlhokahalo ea kliniki ea ho laola boleng ba sampole.