Litaba

Setsi sa tlhahlobo se na le lihlopha tse peli tsa liteko:

Ho e 'ngoe ea tsona, pente e mebala ea burgundy e nang le li-antigens tsa enfelopo ea Novel coronavirus e kopantsoeng le khauta ea Colloid (Novel coronavirus conjugates), 2) lesela la nitrocellulose le nang le mela e' meli ea liteko (mela ea IgG le IgM) le mohala oa taolo (C1 line) .

Mohala oa IgM o kentsoe pele ka letoeba la antihuman IgM antibody, mohala oa IgG o koahetsoe ka Mouse anti-Human IgG antibody.Ha palo e lekaneng ea mohlala oa teko e kengoa ka har'a seliba sa sampole ea sesebelisoa sa tlhahlobo, mohlala ona o falla ka tšebetso ea capillary ho pholletsa le sesebelisoa.IgM anti-Novel coronavirus, haeba e le teng ka har'a mohlala, e tla hokahana le likhokahano tsa Novel coronavirus.

The immunocomplex e ntan'o hapuoa ke reagent e kentsoeng pele ho sehlopha sa IgM, e etsa mohala oa burgundy oa IgM, o bonts'ang sephetho sa tlhahlobo e ntle ea Novel coronavirus IgM.IgG anti-Novel coronavirus haeba e le teng ka har'a mohlala e tla tlama ho likhokahano tsa Novel coronavirus.The immunocomplex e ntan'o hapuoa ke reagent e koahetsoeng moleng oa IgG, e etsa mohala oa IgG oa 'mala oa burgundy, o bonts'ang sephetho sa tlhahlobo ea Novel coronavirus IgG.Ho ba sieo ha mehala ea T (IgG le IgM) ho fana ka maikutlo a sephetho se fosahetseng.E le hore e sebetse e le taolo ea mekhoa, mohala o mebala o tla lula o hlaha sebakeng sa taolo e bontšang hore molumo o nepahetseng oa mohlala o kentsoe 'me ho se ho e-na le li-membrane wicking.

Karolong e 'ngoe, sekhahla sa liteko se entsoe ka likarolo tse latelang: e leng sampole pad, reagent pad, membrane ea reaction, le pad e monyang.The reagent pad e na le colloidal-gold conjugated le monoclonal antibodies khahlano le nucleocapsid protein ea SARS-CoV-2;lera la karabelo le na le li-antibodies tsa bobeli tsa protheine ea nucleocapsid ea SARS-CoV-2.Lesela lohle le tsitsitse ka har'a sesebelisoa sa polasetiki.Ha sampole e eketsoa ka har'a sampole selibeng, li-conjugates tse omisitsoeng ka har'a sebaka sa reagent lia qhibiliha ebe li falla hammoho le sampole.Haeba SARS-CoV-2 antigen e hlahisa sampole, motsoako o entsoeng lipakeng tsa anti-SARS-2 conjugate le vaerase o tla tšoaroa ke li-anti-SARS-2 monoclonal antibodies tse koahetsoeng sebakeng sa liteko (T).Ho ba sieo ha mohala oa T ho fana ka maikutlo a phello e mpe.Ho sebetsa e le mokhoa oa ho laola mohala o mofubelu o tla lula o hlaha sebakeng sa taolo ea taolo (C2) e bontšang hore molumo o nepahetseng oa sampuli o kentsoe 'me ho etsoa ha membrane wicking.